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opuBD [2018-02-13T08:21:59.000Z]
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opuBD [2018-02-13T08:21:59.000Z]

choline and arsenocholine [SW|ABC transporter] (membrane protein)
locus
BSU33700
pI
10.06
mw
23.78 kDa
protein length
226 aa Sequence Blast
gene length
678 bp Sequence Blast
function
compatible solute transport
product
choline and arsenocholine [SW|ABC transporter] (membrane protein)
essential
no
synonyms
proZ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,459,806 → 3,460,486

    The protein

    Catalyzed reaction/ biological activity

  • uptake of choline and arsenocholine [pubmed|29159878]

    • Protein family

  • CysTW subfamily (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|76C8D6BF2CA1FA5E7392C52FFD55FD01EF5AB9DC|OpuCD]

    • [SW|Localization]

  • cell membrane [Pubmed|10092453]

    • Expression and Regulation

    Operons

    genes
    [gene|3F502A4DCB1DAD7C3F968465B13C35213240515B|opuBA]-[gene|70B45E7428DB1F6AEF7D2FE3062CC86BE96674FC|opuBB]-[gene|C48798FE13B1521236F5BC5786B9A02D7BC9A336|opuBC]-[gene|1532EC3D741C5AB3D0B642741218FAE00AD460FA|opuBD]
    description
    [Pubmed|10216873]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10216873], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|5C398C1181CE25CAC079AFBA5871C0B72AE1AA96|GbsR]: repression, (transcriptional roadblock) [Pubmed|32849357,22408163], in [regulon|5C398C1181CE25CAC079AFBA5871C0B72AE1AA96|GbsR regulon]
  • [protein|9F32E96AC9C32832C2F10FB125DDDC50EC9B546B|OpcR]: repression, (sterical interference with RNA polymerase access) [Pubmed|32849357,23960087], in [regulon|9F32E96AC9C32832C2F10FB125DDDC50EC9B546B|OpcR regulon]
  • [protein|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA]: activation, [Pubmed|23646920], in [regulon|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA regulon]

    • regulation

  • induced by choline ([protein|5C398C1181CE25CAC079AFBA5871C0B72AE1AA96|GbsR]) [Pubmed|22408163]
  • expression increases with increasing salinity [Pubmed|32849357]

    • additional information

  • [gene|6FCF6F71C5A8B2345F42BD0C3345C520FD40358B|S1290] acts as [SW|ncRNA|antisense RNA] for the [gene|3F502A4DCB1DAD7C3F968465B13C35213240515B|opuBA]-[gene|70B45E7428DB1F6AEF7D2FE3062CC86BE96674FC|opuBB]-[gene|C48798FE13B1521236F5BC5786B9A02D7BC9A336|opuBC]-[gene|1532EC3D741C5AB3D0B642741218FAE00AD460FA|opuBD] operon [PubMed|,32373088,20525796]
  • expression starts with a delay after osmotic upshift due to transcriptional interference of [SW|RNA polymerase] complexes driving synthesis of the converging opuB and [gene|6FCF6F71C5A8B2345F42BD0C3345C520FD40358B|S1290] mRNAs [pubmed|32373088]
    • view in new tab

    Biological materials

    Mutant

  • BKE33700 (Δ[gene|1532EC3D741C5AB3D0B642741218FAE00AD460FA|opuBD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTCATTGACCTCTTCC, downstream forward: _UP4_TAGAATAGAAAAAGAGGCTG
  • BKK33700 (Δ[gene|1532EC3D741C5AB3D0B642741218FAE00AD460FA|opuBD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTCATTGACCTCTTCC, downstream forward: _UP4_TAGAATAGAAAAAGAGGCTG

    • References

    Reviews

  • 27935846

    • Original publications

  • 10092453,9925583,10216873,21296969,20525796,22408163,23960087,23646920,29159878